Table 5.
Proteins down-regulated by glucose
|
A | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Name | 115:113 | P | 115:114 | P | 116:113 | P | 116:114 | P | Ave | SD | t-test |
| Hxk1p, Hexokinase A |
0.3908 |
0.0002 |
0.4529 |
0.0025 |
0.5200 |
0.0015 |
0.5970 |
0.0181 |
0.4902 |
0.0768 |
0.0014 |
| Pgi1p, Phosphoglucose Isomerase |
0.6792 |
0.0267 |
0.4529 |
0.0042 |
0.7178 |
0.2011 |
0.4613 |
0.0416 |
0.5778 |
0.1215 |
0.0092 |
| Pgm2p, Phosphoglucomutase |
0.4875 |
0.0137 |
0.4246 |
0.0068 |
0.6918 |
0.0936 |
0.6026 |
0.0476 |
0.5516 |
0.1031 |
0.0048 |
| Fbp1p, Fructose-1,6-Bisphosphatase |
0.1528 |
0.0001 |
0.1259 |
0.0001 |
0.0731 |
0.0001 |
0.0625 |
0.0001 |
0.1036 |
0.0372 |
0.0001 |
| Icl1p, Isocitrate Lyase |
0.3281 |
0.0001 |
0.2630 |
0.0001 |
0.2630 |
0.0001 |
0.2128 |
0.0001 |
0.2667 |
0.0409 |
0.0001 |
| Mls1p, Malate Synthase |
0.5395 |
0.0062 |
0.4285 |
0.0019 |
0.7656 |
0.05704 |
0.5916 |
0.0175 |
0.5813 |
0.1216 |
0.0094 |
| Ach1p, Acetyl CoA Hydrolase |
0.4831 |
0.0021 |
0.5152 |
0.0036 |
0.6546 |
0.0213 |
0.6855 |
0.0513 |
0.5846 |
0.0869 |
0.0037 |
| Atp2p, Mitochondrial ATP Synthase Subunit Beta |
0.4966 |
0.0059 |
0.2938 |
0.0053 |
0.6252 |
0.0219 |
0.3597 |
0.0200 |
0.4438 |
0.1277 |
0.0048 |
| Om45p, Mitochondrial Outer Membrane Protein |
0.4613 |
0.0045 |
0.5702 |
0.0144 |
0.4656 |
0.0020 |
0.5649 |
0.0064 |
0.5155 |
0.0521 |
0.0005 |
| Hsp12p, Heat Shock Protein |
0.3565 |
0.0091 |
0.3251 |
0.0945 |
0.2630 |
0.0020 |
0.2466 |
0.0126 |
0.2978 |
0.0448 |
0.0001 |
| Hsp26p, Heat Shock Protein |
0.5445 |
0.0130 |
0.5861 |
0.0162 |
0.3698 |
0.0013 |
0.4018 |
0.0016 |
0.4756 |
0.0916 |
0.0022 |
| Hsp30p, Heat Shock Protein |
0.3981 |
0.0922 |
0.3221 |
0.0446 |
0.2312 |
0.0393 |
0.1905 |
0.0175 |
0.2479 |
0.0550 |
0.0006 |
|
B | |||||||||||
|
Accession # |
Name |
Peptides (95%) |
113:114 |
P |
115:116 |
P |
t-test |
||||
| gi|6321184 |
Hxk1p, Hexokinase A |
20 |
1.3804 |
0.7068 |
0.9462 |
0.9299 |
0.5894 |
||||
| gi|6319673 |
Pgi1p, Phosphoglucose Isomerase |
26 |
0.6368 |
0.3942 |
0.9204 |
0.2883 |
0.3626 |
||||
| gi|817863 |
Pgm2p, Phosphoglucomutase |
18 |
0.8241 |
0.7017 |
0.6730 |
0.2974 |
0.1858 |
||||
| gi|6323409 |
Fbp1p, Fructose-1,6-Bisphosphatase |
7 |
0.8166 |
0.1819 |
1.9953 |
0.1888 |
0.6160 |
||||
| gi|6320908 |
Icl1p, Isocitrate Lyase |
14 |
0.7870 |
0.2416 |
1.2589 |
0.1990 |
0.9383 |
||||
| gi|6324212 |
Mls1p, Malate Synthase |
13 |
0.7586 |
0.5169 |
0.7112 |
0.2358 |
0.0568 |
||||
| gi|6319456 |
Ach1p, Acetyl CoA Hydrolase |
16 |
1.0375 |
0.6989 |
0.7311 |
0.1846 |
0.5882 |
||||
| gi|84028178 |
Atp2p, Mitochondrial ATP Synthase Subunit Beta |
20 |
0.6081 |
0.9613 |
0.8472 |
0.4900 |
0.2633 |
||||
| gi|730224 |
Om45p, Mitochondrial Outer Membrane Protein |
17 |
1.2023 |
0.5644 |
0.9908 |
0.6866 |
0.5289 |
||||
| gi|836740 |
Hsp12p, Heat Shock Protein |
35 |
0.9462 |
0.2305 |
1.3428 |
0.4280 |
0.5991 |
||||
| gi|6319546 |
Hsp26p, Heat Shock Protein |
41 |
1.0965 |
0.8963 |
1.4859 |
0.1689 |
0.3752 |
||||
| gi|6319869 | Hsp30p, Heat Shock Protein | 6 | 0.7943 | 0.4318 | 1.7701 | 0.5325 | 0.6662 | ||||
(A) Proteins that decreased their relative levels in response to glucose re-feeding. Average ratios (Avg) and standard deviations (SD) are given, along with the p-values (P) calculated for each protein's ratio by ProteinPilotTM 4.0 based on the ratios of each identified peptide. A one-sample Student's t-test was used to confirm that each protein's set of ratios for all four comparisons was significantly different from 1 and all p-values are <0.01.
(B) Ratios of 113:114; starved:starved and 115:116; re-fed:re-fed with the number of distinct peptides having at least 95% confidence and a p-value (P) greater than 0.05 calculated for each protein's ratio by ProteinPilotTM 4.0 based on the ratios of each identified peptide. A one-sample Student's t-test was used to confirm that each protein's set of ratios for both comparisons was not significantly different from 1 and all p-values are >0.05.