Table 7.

Proteins that were up-regulated and down-regulated by glucose in our study and the comparison with the studies by Kolkman et al.[9]and Pham et al. [15]

Function	Up-regulated in our study	Kolkman et al.[9]	Pham et al.[15]
Ribosome	Rps0bp, 40S Ribosomal Subunit	identified	not identified
Ribosome	Rps8bp, 40S Ribosomal Subunit	identified	not identified
Ribosome	Rpl3p, 60S Ribosomal Subunit	identified	not identified
Ribosome	Rpl7bp, 60S Ribosomal Subunit	not identified	not identified
Translation	Tef2p, Translational Elongation Factor EF-1 alpha	not identified	Down-regulated in high glucose
Translation	Tef4p, Translational Elongation Factor eEF1B	identified	not identified
Translation	Lia1p, Deoxyhypusine Hydroxylase	identified	not identified
Nutrient Uptake	Pma1p, Plasma Membrane H + −ATPase	Down-regulated under carbon limitation	not identified
	Down-regulated in our study
Glycolysis	Hxk1p, Hexokinase A	Up-regulated under carbon limitation	Up-regulated in high glucose
Glycolysis	Pgi1p, Phosphoglucose Isomerase	identified	Up-regulated in high glucose
Glycolysis	Pgm2p, Phosphoglucomutase	not identified	Up-regulated in high glucose
Gluconeogenesis	Fbp1p, Fructose-1,6-Bisphosphatase	not identified	not identified
Gluconeogenesis	Icl1p, Isocitrate Lyase	identified	not identified
Gluconeogenesis	Mls1p, Malate Synthase	Up-regulated under carbon limitation	not identified
Acetate Metabolism	Ach1p, Acetyl CoA Hydrolase	Up-regulated under carbon limitation	not identified
Mitochondrial ATPase	Atp2p, Mitochondrial ATP Synthase Subunit Beta	identified	not identified
Mitochondrial protein	Om45p, Mitochondrial Outer Membrane Protein	Up-regulated under carbon limitation	not identified
Heat Shock Protein	Hsp12p, Heat Shock Protein	not identified	Down-regulated in high glucose
Heat Shock Protein	Hsp26p, Heat Shock Protein	Up-regulated under carbon limitation	Down-regulated in high glucose
Heat Shock Protein	Hsp30p, Heat Shock Protein	not identified	not identified

Kolkman et al examined steady state expression of proteins in cells grown in two different conditions [9]. Carbon-limited medium contained 19 mM (NH₄)₂SO₄ and 42 mM glucose. Nitrogen limited medium contained 7.5 mM (NH₄)₂SO₄ and 330 mM glucose. Hxk1p, Ach1p, Mls1p, Om45p, and Hsp26p were up-regulated under carbon limitation, whereas Pma1p was down-regulated under carbon limitation. Pgi1p, Icl1p, and Atp2p were identified but showed no significant change in carbon limitation versus nitrogen limitation.

Pham et al examined steady state levels of proteins in cells grown in 120 g/L (normal) to 210 g/L and 300 g/L (high) concentrations of glucose for 68 hours [15]. Hxk1p, Pgi1p, and Pgm2p were up-regulated in high glucose, whereas Tef2p, Hsp12p, and Hsp26p were down-regulated by high glucose.