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. Author manuscript; available in PMC: 2013 Mar 26.
Published in final edited form as: J Mol Biol. 2010 Jul 21;402(1):8–16. doi: 10.1016/j.jmb.2010.07.018

Figure 4. GerBC does not interact with GerD.

Figure 4

A reciprocal Ni2+-NTA affinity pulldown assay was used to characterize the interaction of the GerBC with GerD. Indicated proteins were incubated and reactions were precipitated with Ni2+-NTA resin. The unbound (U) and eluted bound (B) fractions were analyzed by SDS-PAGE and Coomassie staining.