Table 1.

Antibodies Used for Immunohistochemical Analysis

Antigen	Host and Isotype	Source	Antigen Retrieval	Blocking	Dilution
AID	Rat IgG2b	EK2-5G9; Cell Signaling Technology (Beverly, MA)	10 mM citrate pH 61	Strpt/biotin/DAKO serum-free protein block 30 min	1:450
Bcl6	Mouse IgG1	ab17249; Abcam (Cambridge, MA)	1 mM EDTA pH 8.12	Standard blocking3	1:10
CCL21	Goat IgG	AF366; R&D Systems, (Minneapolis, Mn)	10 mM citrate pH 62	Standard blocking3	1:200
CD3	Rabbit IgG	ab16669; Abcam	10 mM citrate pH 62	Standard blocking3	1:500
CD20	Mouse IgG2a	CD20cy; DAKO(Carpinteria, CA)	10 mM citrate pH 62	Standard blocking3	1:1000
CD83	Mouse IgG2b	HB15a; Beckman-Coulter (Brea, CA)	0.1% trypsin in PBS 37°C 30 min	Standard, but O/N at 4°C	1:500
CXCL13	Goat IgG	AF801 R&D Systems	10 mM citrate pH 62	Standard blocking3	1:300
CNA.42 FDC antigen	Mouse IgM	CNA.42; Cell Marque, (Rocklin, CA)	10 mM citrate pH 62	Standard blocking3	1:200
PNAd	Rat IgM	MECA-79; Novus Biologicals (Littleton, CO)	10 mM citrate pH 62	Standard blocking3	1:1000
Podoplanin	Mouse IgG1	D2-40; Vector Labs (Burlingame, CA)	10 mM citrate pH 62	Standard blocking3	1:100

¹95°C bath 45 min, followed by cooling at room temperature for 15 min.

²Boiling water bath for 20 min, followed by cooling at room temperature for 30 min.

³1% BSA/PBS briefly, then 5% normal serum/1% BSA/PBS for 1 h, followed by 15 min streptavidin and biotin blocking steps.