Table 5.
Phagocytosis activity pre and post ozone exposure
| Phagocytosis | R pre ozone | R post ozone | R ozone response* | NR pre ozone | NR post ozone | NR ozone response* | Pre exposure R vs NR | Post exposure R vs NR | Ozone response R vs NR |
|---|---|---|---|---|---|---|---|---|---|
|
Mac (MFI) |
443 (140) |
660 (138) |
139 (59) |
412 (167) |
555 (234) |
91 (50) |
ns |
ns |
ns |
|
% Mac |
34 (4) |
35 (4) |
17 (15) |
51 (6) |
44 (8) |
−14 (13) |
p = 0.03 |
ns |
ns |
|
Particles/Mac |
3 (1) |
4 (1) |
138 (59) |
3 (1) |
4 (2) |
91 (50) |
ns |
ns |
ns |
|
% Internalized Mac |
31 (5) |
32 (4) |
26 (19) |
64 (10) |
57 (17) |
−19 (21) |
p = 0.02 |
ns |
ns |
|
Internal:External Particle Ratio Mac |
0.6 (0.2) |
0.5 (0.1) |
40 (27) |
2.1 (0.6) |
2.3 (0.9) |
−7 (42) |
p = 0.02 |
ns |
ns |
|
PMN (MFI) |
565 (144) |
542 (105) |
61 (41) |
197 (62) |
226 (27) |
78 (62) |
p = 0.03 |
p = 0.01 |
ns |
|
% PMN |
26 (4) |
40 (6) |
70 (26) |
43 (5) |
33 (2) |
−18 (14) |
p = 0.01 |
ns |
p = 0.01 |
|
Particles/PMN |
4 (1) |
4 (1) |
66 (44) |
1 (0.3) |
2 (0.2) |
93 (58) |
p = 0.03 |
p = 0.02 |
ns |
|
% Internalized PMN |
29 (4) |
46 (5) |
95 (37) |
72 (15) |
65 (14) |
−5 (13) |
p = 0.03 |
ns |
p = 0.03 |
| Internal: External Particle Ratio PMN | 0.5 (0.1) | 1 (0.2) | 196 (73) | 6 (3) | 3 (2) | −6 (45) | p = 0.03 | ns | p = 0.04 |
Mean (+/− standard error) values are displayed for each phagocytic indicator.
R = Responders; NR = Non-Responders; MFI = mean fluorescence intensity; Mac = macrophage; PMN = polymorphonuclear neutrophil; ns = not significant.
* Ozone Response values were calculated as the mean of the sum of the individual subject differences in phagocytosis values between post and pre exposure.pre = 48 hours pre exposure; post = 5 hours post exposure.