Figure 7.

IGF1 or IGF2 significantly protects neurons from cytokine-induced toxicity. Mixed human fetal neuronal glial cultures were stimulated with IL-1/IFNγ for 3 days to induce neurotoxicity. A subset of cultures was pre-treated with either IGF1 or IGF2 at 10 ng/ml. Neuronal death was evaluated using viral dye exclusion (A) or MAP2 immunostain (B,C) as previously described [37]. IGF1 or IGF2 reversed the neuronal dead caused by IL-1/IFNγ Results are mean ± SD from triplicate cultures, and are representative of five independent experiments with similar results. One-way ANOVA with Dunnett’s multiple comparison tests. *P < 0.05, ** P < 0.01, *** P < 0.001.