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. 2013 Mar 12;10:37. doi: 10.1186/1742-2094-10-37

Figure 7.

Figure 7

IGF1 or IGF2 significantly protects neurons from cytokine-induced toxicity. Mixed human fetal neuronal glial cultures were stimulated with IL-1/IFNγ for 3 days to induce neurotoxicity. A subset of cultures was pre-treated with either IGF1 or IGF2 at 10 ng/ml. Neuronal death was evaluated using viral dye exclusion (A) or MAP2 immunostain (B,C) as previously described [37]. IGF1 or IGF2 reversed the neuronal dead caused by IL-1/IFNγ Results are mean ± SD from triplicate cultures, and are representative of five independent experiments with similar results. One-way ANOVA with Dunnett’s multiple comparison tests. *P < 0.05, ** P < 0.01, *** P < 0.001.