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Mitotic metaphase chromosomes of Oplegnathus fasciatus males with an X1X2Y sex chromosome system, hybridized with different labeled microsatellite-containing oligonucleotides. Chromosomes were counterstained with DAPI (blue) and microsatellites probes were directly labeled with Cy3 during synthesis (red signals). Letters mark the Y chromosomes. Bar = 5 μm.
