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. 2013 Apr;182(4):1379–1390. doi: 10.1016/j.ajpath.2012.12.032

Figure 1.

Figure 1

Primary RGC cultures express VEGF-A and its receptors. A: Real-time qPCR analysis of VEGFR-2, VEGFR-1, and VEGF-A. Relative expression levels of VEGFR-1 and VEGFR-2 were significantly elevated after VEGF164 treatment compared with control, whereas PlGF had no effect. In contrast, VEGF-A RNA levels were slightly attenuated with VEGF164 supplementation, and enhanced in response to PlGF-1. **P < 0.01, ***P < 0.001 (N = 6). Data are given as means ± SD. B: Immunolabeling of VEGFR-2 (green) and βIII-tubulin (red) in RGCs cultured in control medium or with 2.5 nmol/L PlGF-1, PlGF-2, VEGF120, VEGF164, or VEGF- E for 5 days. Original magnification, ×63. VEGFR-2 immunoreactivity increased after VEGF120, VEGF164, or VEGF-E supplementation, with punctate staining observed both perinuclearly and on neurites. In contrast, VEGFR-2 expression did not increase and remained perinuclear in control and PlGF-1– and PlGF-2–treated RGCs. Scale bar = 10 μm.