Figure 4.

GALA1 patterns. Galanin immunostaining patterns in different CNS regions of control animals (A to F). (A) Synaptic like punctiform pattern in the medulla oblongata, note the perineuronal arrangement (arrowhead). (B) Cytoplasmic immunostaining of oligodendrocyte rows in the cerebellar white matter (arrowheads). (C) Synaptic pattern in the cervical spinal cord dorsal horn (Substantia gelatinosa). (D) Cytoplasmatic immunostaining of astrocytes in the mesencephalon. (E) Intracytoplasmic immunostaining in multiple cells in the Thalamus, either glial cells or small neurons. (F) The neurons and glial cells in the area postrema are strongly immunostained. (G) GALA1 immunolabeling in the medulla oblongata in the control animals (H) clinical scrapie-infected animals. (I) GALA1 immunolabeling in the cerebellar cortex in the control animals (J) clinical scrapie-infected animals. GALA1 immunolabeling was slightly more intense in the cerebellar cortex on the clinical scrapie-infected sheep compared to the control. The remaining regions show little variation.