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. 2013 Jan 30;14:63. doi: 10.1186/1471-2164-14-63

Figure 4.

Figure 4

siRNAs derived from the endogenous CHS-A gene and the CHS-A transgene. Sense strand 21-nt siRNAs that have nucleotide sequences specific to either the endogenous CHS-A gene (CHS-A endogene) or the CHS-A transgene in the vicinity of translational termination codon (indicated by red boxes) are shown. Nucleotide sequences are described in the 5′ to 3′ direction from left to right. Read numbers of siRNAs are shown to the left. Nucleotide positions in the CHS-A endogene are shown at the top. siRNA sequences common to the CHS-A endogene and CHS-A transgene are colored in purple, and those specific to the CHS-A endogene and CHS-A transgene are colored in pink and blue, respectively. Similar patterns were also observed for antisense strand in J-type. No siRNA was detected in this region in wild-type V26 plants (data not shown).