Table 1.
A list of the Salmonella primers used in this study
| Gene | Forward sequence (5′-3′) | Reverse sequence (5′-3′) | Locus or reference | Technique |
|---|---|---|---|---|
|
pflB |
GAAGGTAGGTGTTACATGTCCGAGCTT AATGAAAAGTTAGCCACAGTGCAGGCT GGAGCTGCTTC |
TTTCAGTCAAACCCATTACAT GGTCTGCGTGAAGGTACGAGT AATCATATGAATATCCTCCTTAG |
STM0973 |
Lambda red |
|
adhE |
TATCAGGAGAGCATTATGGCTGTTACT AATGTCGCTGAACTTAACGTGCAGGCT GGAGCTGCTTC |
AAAACAGATAACGAATTAAGC GGATTTTTTCGCTTTTTTCTC TGCCATATGAATATCCTCCTTAG |
STM1749 |
|
| 16S rRNA |
AGGCCTTCGGGTTGTAAAGT |
GTTAGCCGGTGCTTCTTCTG |
[49] |
qPCR |
|
hilA |
ATAGCAAACTCCCGACGATG |
ATTAAGGCGACAGAGCTGG |
[50] |
|
|
hilC |
CTCACCTCTTCAGCGGCCAGT |
CACCCGCAAATGGTCACAGGCT |
STM2867 |
|
|
hilD |
GGTAGTTAACGTGACGCTTG |
GATCTTCTGCGCTTTCTCTG |
[50] |
|
|
invC |
TCGGTCGATCGCTGCAA |
CCCTGGTCGCGAAAATATTC |
STM2894 |
|
|
invE |
CGAATGACGCCAGCTGTTC |
TGCGTCAGGCGTCGTAAA |
STM2897 |
|
|
invF |
TGAAAGCCGACACAATGAAAAT |
GCCTGCTCGCAAAAAAGC |
STM2899 |
|
|
invH |
GGTGCCCCTCCCTTCCT |
TGCGTTGGCCAGTTGCT |
STM2900 |
|
|
invJ |
CGCCGCCGGTTAATTG |
GCTTCCGCTGCAACCAA |
STM2892 |
|
|
orgA |
AGGCAGGGAGCCTTGCTT |
CCCTGATGCATTGCCAAAA |
STM2870 |
|
|
orgB |
ACCATCCCGAAACGCTTTTA |
TTGCCCCTCAGGCTTATCG |
STM2869 |
|
|
prgH |
TGAACGGCTGTGAGTTTCCA |
GCGCATCACTCTGACCTACCA |
STM2874 |
|
|
prgJ |
GGTCTATGGAAACGGACATTGTC |
CGCCGAACCAGAAAAAGC |
STM2872 |
|
|
prgK |
GGGTGGAAATAGCGCAGATG |
TCAGCTCGCGGAGACGATA |
STM2871 |
|
|
rtsA |
ACCCGTGGTGAGCTTGATGAGT |
CCTGTCCAGGTGGGGAGCAT |
SEE_03946 |
|
|
sicA |
GATGAGTCTCTGCGGGCAAA |
GCTCTGTCTCCGCCGTTTT |
STM2886 |
|
|
sicP |
AGATGATATCTGGTTATTGAACGGTATG |
CTGCCGCCAGATAGAATCG |
STM2879 |
|
|
sipA |
CGTCTTCGCCTCAGGAGAAT |
TGCCGGGCTCTTTCGTT |
STM2882 |
|
|
sipB |
GGCGCGCTGCTAACCAT |
TCGCCCCACCGGTAAAA |
STM2885 |
|
| sipC | ATCAGGCTGGTCGATTTACG | GTACGCCGCTACTCAGGAAC | [50] |