Table 3.
Comparison of Gene Expression Profiles of Resting CD4+ T-Cells in the Presence and Absence of MSCs Shows Upregulation of Different Genes Involved in T-Cell Activation
| Gene symbol | Fold regulation | p-Value |
|---|---|---|
| STAT1 | 24.761 | 0.00001 |
| LAG3 | 22.7848 | 0.049585 |
| CD86 | 18.5498 | 0.018956 |
| IL2RA | 14.9631 | 0.0069 |
| GAPDH | 11.7669 | 0.036322 |
| SOCS1 | 11.6857 | 0.004475 |
| TNFSF4 | 9.602 | 0.007012 |
| IRF1 | 8.0371 | 0.000742 |
| CD80 | 8.0185 | 0.000198 |
| TNFRSF9 | 7.4643 | 0.04532 |
| CEBPB | 6.9323 | 0.042343 |
| IL18R1 | 6.0629 | 0.000919 |
| CTLA4 | 5.3889 | 0.009356 |
| TMED1 | 4.2183 | 0.024149 |
| ACTB | 4.084 | 0.000995 |
| JAK2 | 3.7668 | 0.000116 |
| IL15 | 3.5801 | 0.014315 |
| HPRT1 | 3.4742 | 0.023748 |
| IL1R2 | 3.2117 | 0.007129 |
| IL4R | 3.0244 | 0.001042 |
| MAF | 2.9349 | 0.001968 |
| B2M | 2.8154 | 0.001036 |
| CCR4 | 2.6147 | 0.007229 |
| ICOS | 2.2191 | 0.016985 |
| NFATC1 | 2.1936 | 0.045864 |
| NFATC2IP | 2.0515 | 0.011964 |
| STAT4 | 2.0093 | 0.011348 |
| STAT6 | 1.9725 | 0.044474 |
| GLMN | 1.8965 | 0.039781 |
| NFATC2 | 1.8108 | 0.014851 |
| YY1 | 1.8067 | 0.033787 |
| GATA3 | 1.7132 | 0.002088 |
| TFCP2 | 1.6857 | 0.009999 |
| PTPRC | 1.5692 | 0.029901 |
| TYK2 | 1.5227 | 0.008305 |
| IL6R | −1.5227 | 0.036303 |
Resting CD4+ T-cells were cultured in the presence and absence of MSCs, and harvested after 6 days. Gene expression profiles were generated by PCR array (Human Th1-Th2-Th3 RT2 Profiler PCR Array from Qiagen). Resting CD4+ T-cells were used as the control group. The class comparison analysis shows that the presence of MSCs upregulated several genes involved in T-cell activation. Downregulated genes are shown in italics. Only genes showing a fold-regulation >1.5 with a p-value<0.05 were considered in the analysis.