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. Author manuscript; available in PMC: 2013 Mar 26.
Published in final edited form as: Brain Behav Immun. 2011 Jul 21;25(8):1716–1724. doi: 10.1016/j.bbi.2011.07.227

Figure 3. Activation of protein kinase A (PKA) attenuates mouse interferon (mIFN)-alpha-induced phosphorylated signal transducer and activator of transcription (pSTAT)5.

Figure 3

Wild type HT22 cells were grown in 100mm culture dishes until 80% confluent before treatment with a combination of forskolin (FSK)(10 μM) and/or the selective PKA antagonist H89 (10 μM) for 24 hours before treatment with mIFN-alpha for either 0, 5, 15, 30, or 60 minutes. At the end of these time periods cells were processed to obtain whole-cell extracts as described in methods. (A) Representative western blot using an antibody raised against pSTAT5. (B) Optical density analysis of pSTAT5 protein from three independent experiments. * p < 0.05 vs 0 min mIFN-alpha only group; † p < 0.05 vs mIFN-alpha only group at same time point; # p < 0.05 vs mIFN-alpha plus forskolin group at same time point.