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. Author manuscript; available in PMC: 2013 Mar 26.
Published in final edited form as: Brain Behav Immun. 2011 Jul 21;25(8):1716–1724. doi: 10.1016/j.bbi.2011.07.227

Figure 5. Dexamethasone (DEX)-induced luciferase activity in HT22 cells is attenuated by pharmacological antagonism of PKA.

Figure 5

HT22 cells stably transfected with the MMTV-luciferase reporter gene construct were grown in 12-well culture plates until 80% confluent. Cells were then treated with the selective PKA antagonist H89 (0.1, 1, or 10 μM) or vehicle for 22 hours followed by 2 hours treatment with dexamethasone (DEX; 50 nM). Luciferase activity was then measured as described in methods. All conditions were run in triplicate, and values shown are means (±SEM). Results are representative of results obtained in three independent experiments. * p < 0.05 vs DEX only condition; ** p < 0.01 vs DEX only condition.