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. Author manuscript; available in PMC: 2013 Mar 26.
Published in final edited form as: Brain Behav Immun. 2011 Jul 21;25(8):1716–1724. doi: 10.1016/j.bbi.2011.07.227

Figure 7. siRNA directed against PKA enhances inhibition of dexamethasone (DEX)-induced luciferase activity by mouse interferon (mIFN)-alpha in HT22 cells.

Figure 7

HT22 cells stably transfected with the MMTV-luciferase reporter gene construct were grown in 12-well culture plates until 80% confluent. Cells were then transiently transfected for 48 hours with 100 nM siRNA directed against PKA alpha and beta catalytic subunit isoforms. Following transfection, cells were treated with mIFN-alpha (1000 U/ml) for 22 hours followed by DEX (50 nM) for 2 hours. All conditions were run in triplicate, and values shown are means (±SEM). Results are representative of results obtained in three independent experiments. * p < 0.05 vs DEX only condition; † p < 0.05 vs DEX plus mIFN-alpha (no siRNA) condition.