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. Author manuscript; available in PMC: 2014 Mar 14.
Published in final edited form as: Chem Commun (Camb). 2013 Feb 7;49(21):2136–2138. doi: 10.1039/c3cc00181d

Fig. 3.

Fig. 3

Effect of RB-005 on SK1 activity and expression. (A) Concentration-dependent inhibition of SK1 activity by RB-005 using 3 µM sphingosine and 250 µM ATP (results expressed as mean inhibition +/− S.D of control (n = 3, the control is 100% and equals activity against sphingosine alone.). (B) Effects of RB-005 on SK1 down-regulation. PASMC were treated with or without MG132 (10 µM, 30 min) before RB-005 (10 µM, 24 h). Cell lysates were western blotted with anti-SK1 and -actin antibodies. Results are representative of three experiments.