Figure 5. Sugar specifically regulates the expression of MIR156 genes that are important for vegetative phase change.
(A) qRT-PCR of primary MIRNAs in 12-day-old se-1 plants grown in the absence or presence of 10 mM glucose. MIR156A, C, F, and H are repressed by glucose. Asterisk indicates significantly different from the no sugar treatment, p<0.01. (B) The structure of MIR156A-GUS and MIR156C-GUS reporter constructs, and their response to 50 mM glucose or sucrose. Blue indicates the location of GUS, which was inserted in place of the miR156 hairpin. The full-length constructs are approximately 7 kb in length. Solid line: intergenic sequence; dashed line: intron; bar = 2 mm. The staining response was representative of two independent lines, homozygous for a single transgenic insertion. (C) qRT-PCR analysis of the abundance of the MIR156A-GUS, MIR156C-GUS, pri-MIR156A, and pri-MIR156C transcripts in the seedlings illustrated in (B). GUS-fusion transcripts were measured using primers specific for GUS+. Asterisk indicates significantly different from the no sugar treatment, p<0.01.
Figure 5—figure supplement 1. Sucrose represses the expression of the MIR156A-GUS and MIR156C-GUS reporter genes in transgenic seedlings.
