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(A) 3-week-old N. benthamiana plant with agarose gel on petiole stubs. Scale bar = 1 cm. (B) Northern analysis of the level of miR156 in the shoot apex of wounded nondefoliated plants (used as a control), and defoliated plants treated with leaf extract (Ext), or 300 mM of mannitol (Man) or glucose (Glc). miR156 was assayed 3 days after treatment.
