Table II.
Purpose | Sequencea |
---|---|
Amplification of T. forsythia genes for expression with His-tags in B. fragilis | |
tfsA-for | AAATAGATCTGCGGTTTATAAGAGGAAGAAAATAAA |
tfsA-rev | CTTAAGATCTTTTTACACAGCTTTCACTGCATTC |
tfsB-for | CCTCGGATCCATCTCTTGCCTGCCACTCC |
tfsB-rev | CGACGGATCCCTTCACCATCGCTTTTACAGC |
Amplification of B. fragilis genes for expression with His-tags in T. forsythia | |
BF2494-for | ATCAGGATCCACAATCATGAAAAGAGTATTATTTTC |
BF2494-rev | ATCAGGATCCCATCATTTTCTCGATTTCTTCGAATTC |
BF3567-for | ATCAGGATCCTACTAACTAAACGTGATTAATTTATG |
BF3567-rev | ATCAGGATCCACGGGTTACTTCCAAATACTTCACC |
aSequences are given 5′–3′ with restriction sites underlined.