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. 2012 Dec 19;23(5):568–577. doi: 10.1093/glycob/cws172

Table II.

Oligonucleotide primers used in this study

Purpose Sequencea
Amplification of T. forsythia genes for expression with His-tags in B. fragilis
 tfsA-for AAATAGATCTGCGGTTTATAAGAGGAAGAAAATAAA
 tfsA-rev CTTAAGATCTTTTTACACAGCTTTCACTGCATTC
 tfsB-for CCTCGGATCCATCTCTTGCCTGCCACTCC
 tfsB-rev CGACGGATCCCTTCACCATCGCTTTTACAGC
Amplification of B. fragilis genes for expression with His-tags in T. forsythia
 BF2494-for ATCAGGATCCACAATCATGAAAAGAGTATTATTTTC
 BF2494-rev ATCAGGATCCCATCATTTTCTCGATTTCTTCGAATTC
 BF3567-for ATCAGGATCCTACTAACTAAACGTGATTAATTTATG
 BF3567-rev ATCAGGATCCACGGGTTACTTCCAAATACTTCACC

aSequences are given 5′–3′ with restriction sites underlined.