Table III.
Starvation media.
| Medium | Recipe |
|---|---|
| Dryl’sa | 0.59 g of Na citrate-2H2O (2 mM) |
| 0.14 g of NaH2PO4 • H2O (1 mM) | |
| 0.14 g of Na2HPO4 (1 mM) | |
| 0.13 g of CaCl2 (1.5 mM) | |
| (To avoid precipitation of the CA phosphate, the CaCl2 solution is autoclaved separately from the mixture of sodium salts, and the two solutions are mixed aseptically after cooling.) | |
| Tris bufferb | 10 mM Tris HCl, pH 7.5 |
| NKC solutionc | 0.2% NaCl |
| 0.008% KCl | |
| 0.12% CaCl2 |
a
Dryl, S. (1959). Antigenic transformation in paramecium aurelia after homologous antiserum treatment during autogamy and conjugation. J. Protozool., 6(25)
b
Bruns, P. J., and Brussard, T. B. (1974). Pair formation in tetrahymena pyriformis, an inducible developmental system. J Exp Zool, 188(3), 337–44.
c
Sugai, T., and Hiwatashi, K. (1974). Cytologic and autoradiographic studies of the micronucleus at meiotic prophase in tetrahymena pyriformis. J Protozool, 21(4), 542–58.