Table 1.
Sample | Sex | Treatment | CFU number | Organ weight | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Period post-infection | ||||||||||||||
12–24 h | 24–48 h | 12–48 h | 12–24 h | 24–48 h | 12–48 h | |||||||||
WT | KO | WT | KO | WT | KO | WT | KO | WT | KO | WT | KO | |||
Lung | Males | FA | + | − | + | − | + | + | − | + | + | − | + | + |
Ozone | + | + | − | ++ | − | + | ++ | + | ++ | − | + | + | ||
Females | FA | + | − | − | + | + | + | ++ | ++ | − | *↓ | ++ | − | |
Ozone | + | + | + | + | + | + | ++ | ++ | + | + | + | + | ||
Blood | Males | FA | + | + | − | − | + | + | ||||||
Ozone | + | + | − | + | + | + | ||||||||
Females | FA | + | − | + | + | + | + | |||||||
Ozone | + | − | + | + | + | + | ||||||||
Spleen | Males | FA | + | + | − | + | + | + | + | − | − | + | + | + |
Ozone | + | + | + | + | + | + | − | − | ++ | ++ | ++ | + | ||
Females | FA | + | − | + | + | + | + | − | − | + | + | + | + | |
Ozone | + | + | + | + | + | + | − | − | − | − | − | − |
The data from Figs. 1 and 2 were used for the analysis shown in this Table. Comparisons were made between groups of mice of the same type (i.e. wild type (WT) or SP-A (−/−) KO)), and the same sex that were subjected to the same treatment (i.e. to filtered air (FA) or ozone (O3) exposures) during the course of pneumonia (i.e. between the following time points post-infection: 12 and 24 h, 24 and 48 h, and 12 and 48 h). For statistical analysis, log10 transformed data for the CFU numbers were used, and untransformed data were used for the organ weights. Significant differences (p <0.05) were assessed by a t-test (shown with “++”, and by a Mann–Whitney Rank Sum Test (shown with “+”) for the cases where either normality or equal variance tests failed, as suggested by the SigmaPlot 10.0 software. If no significant differences were detected between compared groups, these are indicated with “− ”. In all cases the values for CFU numbers and organ weights were increased with increased time post-infection. However, lung weight of FA-exposed SP-A (−/−) female mice was decreased from 24 to 48 h time point (shown with asterisk).