Skip to main content
. 2013 Apr;345(1):69–75. doi: 10.1124/jpet.112.202481

Fig. 4.

Fig. 4.

Effect of paclitaxel on LPS-induced activation of p65, degradation of IκBa, and expression of TLR4 in HK-2 cells. HK-2 cells were treated with 10 μg/ml LPS in the presence or absence of 2 μM paclitaxel. (A) Cell viability determined by MTT assay at 24 hours of treatment. (B) NF-κB DNA-binding activity determined by ELISA assay at 2 hours of treatment. (C) Immunoblot analysis of phospho-p65, p65, IκBa, and TLR4 expression in HK-2 cells at 2 hours of treatment. (a) Representative immunoblot. (b,c,d,e) Results of densitometric analysis. Data are presented as mean ± S.E.M. (n = 6). △P < 0.05 versus the mock group or paclitaxel-only group; #P < 0.05 versus the LPS group. IκB-α, inhibitor of κB-α.