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. 2013 Apr;345(1):69–75. doi: 10.1124/jpet.112.202481

Fig. 6.

Fig. 6.

Effect of MD-2 knockdown on LPS-induced NF-κB DNA-binding activity and expression of TNF-α and IL-6 levels. HK-2 cells were transfected with MD-2 siRNA or control siRNA, followed by treatment with 10 μg/ml LPS in the presence or absence of 2μM paclitaxel. (A) Immunoblot analysis to verify MD-2 knockdown by a specific siRNA. (B) NF-κB DNA-binding activity determined using ELISA assay. (C and D) TNF-α and IL-1 expression analyzed by ELISA assay. Data are presented as mean ± S.E.M. (n = 6). △P < 0.05 versus the mock group; #P < 0.05, versus the LPS plus control siRNA group.