Fig. 5.
CPA-mediated inhibition of NE oxidation currents and constrictions in sham, but not DOCA-salt MAs. Electrically evoked NE release (60 stimuli, 10 Hz frequency, 0.5 millisecond pulse duration, 60–80 V intensity) was detected by amperometry with a carbon fiber microelectrode. CPA was then added in increasing concentrations. Periarterial sympathetic nerves were stimulated and oxidation currents (A), and constrictions (B) were inhibited by CPA. Duration of nerve stimulation (ns) is indicated by the bars. Imax indicates amplitude of NE oxidation current. (C) The CPA CRC was right shifted in DOCA-salt arteries, compared with sham arteries. (D) There were no differences in CPA CRCs between sham and DOCA-salt veins. Data are mean ± S.E.M. *Significantly different from sham arteries (P < 0.05). Data were analyzed using two-way analysis of variance (ANOVA) and Bonferoni’s post hoc test.