Fig. 1.

Time- and concentration-dependent inactivation of reconstituted human CYP2E1 by PEITC following incubation with NADPH. The incubation conditions and measurement of 7-EFC O-de-ethylation activity were described under Materials and Methods. The concentrations of PEITC that were used were (●) 0, (□) 1, (▴) 2.5, (◊) 5, (○) 10, (▪) 25 and (Δ) 50 μM. The data represent the mean and S.D. from six separate experiments done in duplicate. The inset shows the double-reciprocal plot of the rates of inactivation as a function of the PEITC concentration.