Fig. 6.

ESI LC-MS/MS analysis of phenethylamine with a precursor ion at m/z 122 formed by the metabolism of PEITC by human CYP2E1 in the reconstituted system. CYP2E1 was reconstituted with reductase and incubated with PEITC in the presence or absence of NADPH. Phenethylamine was extracted and analyzed as described under Materials and Methods. Upper panel, extracted ion chromatogram at m/z 122 of the sample incubated with human CYP2E1 and PEITC in the presence NADPH (left panel), and its MS/MS pattern (right panel). Lower panel, extracted ion chromatogram at m/z 122 for the chemical standard sample of phenethylamine (left panel) and its MS/MS pattern (right panel).