Fig. 8.

(A) Effect of PIC on the 7-EFC O-de-ethylation activity of human CYP2E1. Assay conditions were as described in Materials and Methods. The percent of human CYP2E1 7-EFC O-de-ethylation activity remaining 10 minutes after the addition of PIC was plotted against the molar ratio of PIC to CYP2E1. The data shown represent the mean of three separate experiments done in duplicate. (B) Difference binding spectra for the addition of 25 μM PEITC (blue) or PIC (red) to CYP2E1 (1 µM). The conditions for the binding spectra are described in Materials and Methods.