TABLE 1.
Effect of PEITC on the 7-EFC O-de-ethylation activity, P450 concentration, and the amount of heme remaining for human CYP2E1
Assay conditions were as described in Materials and Methods. Samples were first dialyzed before reconstitution with fresh reductase. The values shown represent the means ± S.D. from two experiments done in duplicate.
| % Activity Remaining | % P450Remaining | % Heme Remaining | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| 0 min | 10 min | After Dialysis | FrFresh Reductase | 0 min | 10 min | After Dialysis | 0 Min | 10 Min | After Dialysis | |
| −PEITC, −NADPH | 100 | 91 ± 4 | 100 | 100 | 100 | 96 ± 8 | 100 | 100 | 100 ± 4 | 100 |
| +PEITC,−NADPH | 82 ± 4 | 77 ± 2 | 82 ± 4 | 85 ± 9 | 103 ± 8 | 93 ± 10 | 104 ± 3 | 100 ± 1 | ||
| +PEITC,+NADPH | 83 ± 5 | 19 ± 1 | 27 ± 4 | 26 ± 4 | 44 ± 3 | 42 ± 7 | 70 ± 2 | 64 ± 6 | ||
7-EFC, 7-ethoxy-4-(trifluoromethyl) coumarin; P450, cytochrome P450; PEITC, phenethyl isothiocyanate.