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. Author manuscript; available in PMC: 2013 Mar 26.
Published in final edited form as: Biochemistry. 2011 Aug 15;50(35):7441–7443. doi: 10.1021/bi201098r

Figure 1.

Figure 1

HPLC CPAF assay using Abz-VRLRSSVPGV-NH2: A. Representative HPLC trace showing 1 and the Abz containing hydrolysis product Abz-VRLRS-OH (2) as viewed by fluorescence detection at λem = 420 nm. B. Determination of apparent kinetic parameters for CPAF for 1. Assays were performed in a total volume of 100 μL containing Assay Buffer, CPAF (62.5 nM), and varying concentrations of Abz-VRLRSSVPGV-NH2 (0-6 mM).