Figure 3.

Blocking small ubiquitin‐like modifier (SUMO)1–3 conjugation in F98 rat glioblastoma cells by silencing their expression suppressed DNA synthesis. Exposure to 4‐hydroxytamoxifen (4‐OHT) induced expression of miR‐SUMO (three miRNAs against SUMO1, SUMO2, and SUMO3) and miR‐Neg (control miRNA) in stably transduced cells. Western blot analysis depicts SUMO1 (A) and SUMO2/3 (B) in cells without (−) and with (+) 4‐OHT exposure, showing depletion of SUMO1 and SUMO2/3 in miR‐SUMO expressing cells. Uptake of ³ H‐thymidine as an indicator of DNA synthesis in transduced cells is shown in (C) and suggested that blocking SUMO1–3 conjugation by silencing their expression suppressed DNA synthesis. Data are presented as means ± SD (n = 6/group). Statistical analysis was carried out using anova and Fisher's PLSD test. ***P < 0.001.