FIGURE 3:
Expression of Myo5B-tail impairs stretch-induced exocytosis. (A) Umbrella cells were transduced in situ with adenoviruses expressing GFP (control) or GFP-tagged Myo5B-tail. The excised bladders were mounted in Ussing stretch chambers and either left in a quiescent state (no stretch) or subjected to stretch at t = 0. Changes in capacitance (CT), normalized to the starting value, were recorded. Data are mean ± SEM (n ≥ 6). Values significantly different from control are indicated with an asterisk. (B) Umbrella cells were transduced with adenoviruses encoding human growth hormone (hGH) alone, hGH and GFP, or hGH and Myo5B-tail. The bladders were then filled, and the release of hGH into the bladder lumen was quantified as described in Materials and Methods. Data are mean ± SEM (n ≥ 6). Values significantly different from control are indicated with an asterisk.