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. 2013 Apr 1;24(7):1020–1029. doi: 10.1091/mbc.E12-08-0575

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© 2013 Lu et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 4: — Dispergo blocks the recruitment of VSVG^ts-GFP to the ERES. BSC1 cells were transduced with adenovirus to express VSVG^ts-GFP overnight at 40°C. Cells were treated with either DMSO (control) or dispergo for 40 min (A, C). Subsequently, the temperature was shifted to 32°C for 10 min (40°C→32°C; B, D) before fixation and immunofluorescence detection of endogenous Sec31a. Images were acquired using wide-field fluorescence microscopy. The enlarged boxed regions in the merge images are shown in the bottom left of the corresponding composite images. Arrowheads highlight the colocalization of VSVG^ts-GFP with Sec31a in control. Scale bar, 10 μm.