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. 2013 Apr 1;24(7):1020–1029. doi: 10.1091/mbc.E12-08-0575

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© 2013 Lu et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 6: — Relocalization of the Golgi marker GalT-tomato to the ER induced by dispergo. (A) BSC1 cell transiently expressing GalT-tomato and GFP-Sec61β were treated with dispergo and imaged by spinning disk confocal microscopy (see Supplemental Movie S4). Arrowheads highlight colocalization of GalT-tomato and GFP-Sec61β at ER patches marked by GFP-Sec61β. Scale bar, 10 μm. (B) Time dependence for the loss of GalT-tomato from the perinuclear region corresponding to the Golgi apparatus induced by dispergo in BSC1 cells stably expressing GalT-tomato. The fluorescence intensity of GalT-tomato at the perinuclear region determined before dispergo addition was used to normalize the data. Plot shows mean values and SD for each time point (n = 13 cells). Golgi t_1/2 represents the mean time ± SD that it took for the signal of GalT-tomato to be reduced by 50%. “ER patches onset” marks the time at which the appearance of ER patches labeled with GFP-Sec61β was first detected (n = 10 cells).