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. 2013 Apr 1;24(7):1068–1082. doi: 10.1091/mbc.E12-09-0661

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© 2013 Waheed et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 7: — Differential role of the GEF-H1 T678 and S885 phosphorylation sites in GEF-H1 activation toward Rac and RhoA. LLC-PK₁ cells were transfected with GFP-tagged wild-type GEF-H1 (GEF-H1^WT) or the nonphosphorylatable point mutant GEF-H1^T678A or GEF-H1^S885A as indicated. At 48 h posttransfection, cells were treated with 10 ng/ml TNF-α (5 min), and activated GEFs were precipitated using RhoA(G17A) (A, C) or Rac(G15A) (B, D). The GFP-tagged GEF-H1 protein was detected by Western blotting using anti-GFP. The blots were quantified as described earlier. The graphs show mean ± SE from n = 4 (A, D) or 3 (B and C) independent experiments.