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Increased Der1-HA levels suppress the CPY* degradation defect in nat3Δ cells. Cells all carried the prc1-1 allele and had the indicated additional chromosomal deletions; the strains were transformed with p415GPD-DER1-HA. The estimated CPY* half-lives were determined by quantitative immunoblotting using antibodies to HA and PGK and normalization to PGK levels. Strains used: prc1–1 hrd1Δ (MHY6855); prc1–1 nat3Δ (MHY6920); and prc1–1 der1Δ (MHY7110).
