TABLE 1:
MS/MS analysis of Der1 N-terminal acetylation in NAT3 and nat3Δ cells.
| WT (MD) Der1 | |||||
|---|---|---|---|---|---|
| Peptide identified by mascot search | Charge state | m/z | MH+ | nat3ΔTIC max | WTTIC max |
| MDAVILNLLGDIPLVTR | 2 | 927.0326 | 1853.0652 | 6.18E+06 | 2.87E+05 |
| MDAVILNLLGDIPLVTR + Met oxidation | 2 | 935.0289 | 1869.0578 | 1.22E+06 | 2.38E+05 |
| MDAVILNLLGDIPLVTR + Met oxidation | 3 | 623.6899 | 1869.0697 | 6.76E+06 | 4.61E+05 |
| MDAVILNLLGDIPLVTR + acetylation | 2 | 948.0381 | 1895.0762 | Not seen | 1.11E+07 |
| MDAVILNLLGDIPLVTR + acetylation | 3 | 632.2623 | 1894.7869 | Not seen | 7.45E+05 |
| MDAVILNLLGDIPLVTR +acetylation + Met oxidation | 2 | 956.0353 | 1911.0706 | Not seen | 8.57E+05 |
| Total TIC (± Met oxidation and ± acetylation) | 1.42E+07 | 1.37E+07 | |||
| Total TIC for acetylated peptides (± Met oxidation) | 0.00E+00 | 1.27E+07 | |||
| Percentage of total peptides acetylated | 0 | 93 | |||