FIGURE 2:

LGN is required for the cortical localization of DYNC1H1 during mitosis. (A) LGN depletion results in reduced cortical localization of DYNC1H1. MDCK cells transduced by control lentivirus (control) or lentivirus expressing shRNAs targeting different regions of LGN (LGN-KD1-7 and LGN-KD2-6) were stained with anti-DYNC1H1 antibody and DNA dye. Bar, 10 μm. (B) Quantitation of the fluorescence intensity of cortical DYNC1H1 from images acquired in A. n = 50 for each set; *p < 0.01. (C) Slight overexpression of LGN leads to enhanced cortical localization of DYNC1H1. Stable Tet-Off MDCK cells expressing Venus-LGN were cultured in the presence (+Dox) or absence (–Dox) of doxycycline. At 24 h later, cells were stained as in A. Bar, 10 μm. (D) Quantitation of the fluorescence intensity of cortical DYNC1H1 from images acquired in C. n = 50 for each set; *p < 0.01.