Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2013 Apr 1;24(7):914–922. doi: 10.1091/mbc.E12-11-0812

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2013 King et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

“ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society of Cell BD; are registered trademarks of The American Society of Cell Biology.

PMC Copyright notice

FIGURE 6: — Hsl7p can be recruited to septin rings even in unbudded cells. Cells marked with both GFP-Hsl7p and the septin Cdc3p-mcherry (DLY15772) were arrested in G1 with pheromone and released into fresh media. Ten minutes later, the cells were placed on agar slabs for imaging. (A) Control cells assembled septin rings, budded, and recruited Hsl7p to the neck. (B and D). Cells placed on slabs containing 100 μM Lat B assembled septin rings but failed to form visible buds. Nevertheless, Hsl7p was recruited to the septin ring (often after a delay). Most cells in this experiment formed septin rings away from the mating projection (B) but some formed rings within the projection (D). Hsl7p was recruited in both cases. Selected frames from the indicated times after plating are shown and marked with arrows indicating initial septin ring assembly (Cdc3p-mCherry) or initial Hsl7p recruitment (GFP-Hsl7p). Prior to its recruitment to the septins, Hsl7p is localized to the spindle pole body (Cid et al., 2001), which appears as a dot. (C) Quantification of the interval between initial septin ring assembly and initial Hsl7p recruitment. Each circle is one cell, and the line marks the average interval. For this analysis, we scored only cells in which septin rings formed away from the mating projection (as in A and B) because, when rings formed within the projection, it was difficult to determine exactly when the ring assembled due to the presence of septins at the base of the projection. The Lat-induced delay was statistically significant (p < 0.001) as assessed by the Student's t test.