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. 2013 Apr 1;24(7):923–932. doi: 10.1091/mbc.E13-01-0034

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© 2013 Bodor et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 1: — H4, but not H3.1, H3.3, or H2B, is coassembled with CENP-A in G1 phase. (A) Outline of quench-chase-pulse labeling strategy, allowing visualization of a newly synthesized pool of SNAP, followed by Triton-based preextraction. (B) Results of A for indicated histone–SNAP fusion proteins. Enlargement to the right shows rescaled images to indicate colocalization of newly synthesized H4-SNAP with centromeres (marked by CENP-C). Enlargements below show single–focal plane images to indicate specific subnuclear assembly patterns. Blue, green, and red arrows show G1, early S, and mid/late S phase cells, respectively. (C) Outline of quench-chase-pulse experiment on synchronized cells. (D) Results of C for SNAP-tagged histone proteins. CENP-C staining indicates centromere positions. Enlargement shows colocalization of newly synthesized H4-SNAP with centromeres.