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. 2013 Apr 1;24(7):923–932. doi: 10.1091/mbc.E13-01-0034

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© 2013 Bodor et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 5: — HJURP is dispensable for stable retention of CENP-A. (A) Outline of quench-chase-pulse and pulse-chase experiment combined with siRNA-mediated protein depletion. Note that quench-chase-pulse and pulse-chase experiments were done in parallel to minimize variation of RNAi efficiency. (B) Results of A after depletion of GAPDH (control), HJURP, or CENP-A. Images are displayed for nascent CENP-A-SNAP and the preincorporated pool 24 h after target protein depletion. (C) Quantification of centromeric TMR-Star fluorescence after depletion of GAPDH (white), HJURP (light gray), or CENP-A (dark gray) for indicated protein pools of CENP-A–SNAP. Results were normalized against control RNAi. Average and SEM for at least three independent experiments. Asterisks and NS, respectively, indicate statistically significant (p < 0.01) and nonsignificant (p > 0.05) differences from control samples in paired t tests.