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. 2013 Apr 1;24(7):945–963. doi: 10.1091/mbc.E12-10-0736

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© 2013 Choi et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 6: — Rap1 and Cno are required for normal Baz localization during gastrulation, but other cues partially restore apical Baz. Genotypes and antigens indicated. (A–L) Baz localization in apical–basal sections through embryos of indicated stages. We cannot distinguish maternal/zygotic and zygotically rescued mutants at this stage; we thus divided embryos into two classes on the basis of phenotypic severity and show representative examples of each class. (A, B, G, H) In WT, Baz is apically localized at gastrulation onset (stage 6; A, G) and tightens up and moves to the extreme apical end of the cell during germband extension (stage 7; B, H). (C, D, I, J) In presumptive Rap1^MZ (C, D) and cno^MZ mutants (I, J), Baz slowly becomes enriched apically but significant mislocalized Baz remains. (E, F, K, L) In maternally mutant but zygotically rescued Rap1^M (E, F) and cno^M (K, L) embryos, restoration of apical Baz proceeds more completely than in maternal/zygotic mutants, but rescue remains incomplete. Scale bars, 10 μm. (M–X) Average image intensity along the apical–basal axis in projected cross-sections was assessed as in Figure 2, and data were displayed either as heat maps illustrating intensity with different colors (left, apical is on top; each column is a different embryo) or graphically, displaying pixel intensity vs. depth from the apical surface (right, each line is a different embryo). Genotypes and stages are indicated. Because this analysis did not allow us to definitively distinguish zygotically rescued embryos, we binned the embryos into the most severe and least severe (they should be present in a 1:1 ratio) and labeled these as presumptive maternal/zygotic or zygotically rescued embryos.