TABLE 1:
Fly stocks, antibodies, and probes.
| Fly stocks | Source | |
|---|---|---|
| Rap1rv(R)B1FRT3L2A/TM3twiGFP | I. Hariharan (University of California, Berkeley, CA) | |
| FRT82B cnoR2/TM3twiGFP | U. Gaul (Ludwig-Maximilians-Universität München, Munich, Germany) | |
| aPKCk06403FRT2R/CyO | C. Doe (University of Oregon, Eugene, OR) | |
| UASbazRNAi (stock # 35002) | Bloomington Drosophila Stock Center (Bloomington, IL) | |
| UASRapRNAi (stock # 35047) | Bloomington Drosophila Stock Center | |
| Antibodies/probes (species) | Dilution (IF) | Source |
| anti-ArmN27A1 (M-IgG2a) | 1:50 | Developmental Studies Hybridoma Bank (University of Iowa, Iowa City, IA) |
| anti-Nrt (M-IgG2a) | 1:100 (heat fixation)/1:25 (4% formaldehyde fixation) | Developmental Studies Hybridoma Bank |
| anti–DE-DCAD2 | 1:100 | Developmental Studies Hybridoma Bank |
| anti-Rap1 (rabbit) | 1:500 | This work |
| anti-Baz (rabbit or guinea pig) | 1:500 | J. Zallen (Memorial Sloan-Kettering Cancer Center) |
| anti-aPKC (rabbit) | 1:1000 | Santa Cruz Biotechnology (Santa Cruz, CA) |
| anti–α-Tubulin (clone YL1/2; rat) | 1:250 | Millipore (Billerica, MA) |
| anti–γ-Tubulin (clone GTU-88; M-IgG1) | 1:500 | Sigma-Aldrich (St. Louis, MO |
| anti-Zipper (myosin II heavy chain; rabbit) | 1:1000 | C. Field (Harvard University, Cambridge, MA) or D. Kiehart (Duke University, Durham, NC) |
| Alexa–phalloidin | 1:500 | Life Technologies (Carlsbad, CA) |
| Secondary antibodies: Alexa 488, 568, and 647 | 1:500 | Life Technologies |