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. 2013 Apr 1;24(7):964–981. doi: 10.1091/mbc.E12-10-0742

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© 2013 Leonhardt et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 5: — Trafficking and processing of selected PMEL NTR point mutants. (A–C) Western blot analysis of SDS-lysed total membranes using PMEL-specific antibodies Pep13h (A), HMB45 (B), and I51 (C). Horizontal dashed lines separate different exposures of the same blot. (D–F) Selected cell lines from A–C and Supplemental Figure S4, A–C, were analyzed by IF using antibodies against newly synthesized (Pep13h) and mature (HMB45) PMEL (D), the early endosomal marker EEA1 (ab70521) and mature PMEL (HMB50; E), or the lysosomal marker LAMP1 (H4A3) and mature PMEL (HMB50;F). The corresponding IF data for the other point mutants generated for this study are shown in Supplemental Figures S4 and S5.