TABLE 1:

Nuclear actin reporter design.

Domain	Description	Selected member(s)
WH2	Actin monomer–binding domain found in nucleators and nucleation-promoting factors	JMY
RPEL	Actin monomer–binding domain found in the MAL family of transcriptional coactivators	MAL
ERM	Actin filament–binding domain commonly found in focal adhesion components	Ezrin, talin
CH	Actin filament–binding domain commonly found in cross-linking proteins	α-Actinin, dystrophin, utrophin
HP	Actin filament–binding domain commonly found in focal adhesion components	ABLIM3
Orphan	Unique actin-binding domains	Abl1 FABD, cortactin FABD, vinculin FABD, Lifeact FABD, VASP GABD

Protein domains tested as candidate nuclear actin reporters. CH, calponin homology; ERM, ezrin/radixin/moesin; HP, headpiece; WH2, WASp-homology 2 domain.