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. 2013 Apr 1;24(7):995–1006. doi: 10.1091/mbc.E12-10-0723

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© 2013 Bliss et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 7: — The knockdown of lasp-2 increases cell spreading rates. (A) siRNA to human lasp-2 was used to reduce lasp-2 RNA and protein levels in HEK 293 cells. RT-PCR shows that lasp-2 transcript is greatly reduced with lasp-2 siRNA. Lasp-2 protein levels are also significantly reduced with lasp-2 siRNA treatment. (B) In cell-spreading assays, the spread area of lasp-2 siRNA–treated cells was ∼15% increased in comparison to controls.