Table 1.
Kinetics of DENV-3 DIII-MPB variants with 8A1.
| DIII Variant | 301 | 383 | ka (105M−1s−1) | kd (10−3s−1) | KD (nM) | t ½ (sec) | FRNT50 (ug/ml) |
|---|---|---|---|---|---|---|---|
| III | T | N | 2.176 | 43.51 | 200.9 | 16.6 | 13.5* |
| I | L | K | 1.124 | 11.51 | 103.2 | 60.5 | 0.20 |
| II | L | K | 0.268 | 12.62 | 470.9 | 59.0 | 0.37 |
| T301L | L | N | 0.6892 | 10.15 | 155.3 | 68.2 | 1.08 |
| N383K | T | K | 0.823 | 59.8 | 718.2 | 11.9 | 3.50 |
| IV | S | K | NA | NA | NA | NA | 413.1 |
There are two genotype III virus clones: SL ’89 and Cuba ’02. FRNT50 of SL ’89 (III) was indicated in this table and FRNT50 of Cuba ’02 (III) is 9.9 ug/ml. 8A1 binding was not detected for genotype IV DIII-MBP at concentrations of 5000 nM.