Skip to main content
. Author manuscript; available in PMC: 2014 Apr 1.
Published in final edited form as: J Immunol. 2013 Mar 1;190(7):3732–3739. doi: 10.4049/jimmunol.1202964

Figure 1. Kinetics of Fab 19 or Fab 19 inferred germline-reversion variant binding to RSV F protein, measured by surface plasmon resonance.

Figure 1

Each variant is labeled to indicate the amino acid position with the somatically mutated residue from the matured antibody (to left) and the inferred germline residue to which it was reverted (to right). Association (A) or dissociation (B) rate of Fab 19 or Fab19 inferred germline-reversion variant binding to RSV F protein. Steady-state affinity [KD] of Fab19 or Fab19 inferred germline-reversion variants for binding to the RSV F protein (C). Each bar represents the mean rate constant or affinity (± standard deviation) calculated from experiments run in triplicate. * Indicates p < 0.05 compared to Fab19, as calculated by an unpaired t test.