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. Author manuscript; available in PMC: 2014 Apr 1.

Published in final edited form as: Drug Deliv Transl Res. 2012 Nov 30;3(2):183–194. doi: 10.1007/s13346-012-0113-z

Fig. 2 — Mammosphere formation assay. (A) Mammosphere formations for different cell lines require different culture conditions. MCF-7 forms mammospheres in DMEM, while MCF-7/Adr and MDA-MB-231 only form mammospheres in K-SFM, signify differences in cellular properties. Surface marker profiling of MCF-7/Adr (B) and MDA-MB-231 (C) in mammosphere culture conditions showed MCF-7/Adr’s asymmetric division. Mammosphere culture condition after 7 days showed significant reversal of CSC phenotypes in MCF-7/Adr (the positive expression of CD44, CD49f and ESA, and the negative expression of CD24). MDA-MB-231, on the contrary, did not show drastic alteration in phenotypes under mammosphere culture condition.

Fig. 2 — Mammosphere formation assay. (A) Mammosphere formations for different cell lines require different culture conditions. MCF-7 forms mammospheres in DMEM, while MCF-7/Adr and MDA-MB-231 only form mammospheres in K-SFM, signify differences in cellular properties. Surface marker profiling of MCF-7/Adr (B) and MDA-MB-231 (C) in mammosphere culture conditions showed MCF-7/Adr’s asymmetric division. Mammosphere culture condition after 7 days showed significant reversal of CSC phenotypes in MCF-7/Adr (the positive expression of CD44, CD49f and ESA, and the negative expression of CD24). MDA-MB-231, on the contrary, did not show drastic alteration in phenotypes under mammosphere culture condition.