Figure 1. Co-immunoprecipitation of DAT and Gβγ subunits.

(A, B) Immunoprecipitation of DAT with two DAT antibodies directed against different DAT epitopes results in the co-precipitation of Gβ subunits from mouse striatum. Control experiments include immunoprecipitations with nonspecific rat or rabbit IgGs or immunoprecipitations from DAT knockout striatum or mouse cerebellum. (C, D) Immunoprecipitation of DAT resulted in the co-immunoprecipitation of Gβ subunits from MN9D-DAT cells or HEK293-DAT cells. Control experiments include immunoprecipitations from MN9D or HEK293 mock-transfected cells. Three different DAT antibodies were used; DAT¹ (Mab369, Millipore), DAT² (H-80, Santa Cruz), and DAT³ (c-20, Santa Cruz). Immunoblotting was performed with a Gβ pan-antibody (T-20, Santa Cruz). Cereb = cerebellum, St = striatum, DAT-KO = DAT knockout.