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. 2013 Mar 26;8(3):e59788. doi: 10.1371/journal.pone.0059788

Figure 2. Direct interaction between Gβγ subunits and the carboxy terminus of DAT.

Figure 2

(A) Schematic representation of DAT depicting cytoplasmic segments used in GST pull-down assays. (B) The carboxy terminus of DAT (GST-DATc) precipitated Gβγ subunits from mouse striata. (C) Immuno-far-western assay showing a direct interaction between GST-DATc and purified Gβγ from bovine brain. RP = Ponceau Red.